MORPHOLOGY
Counter staining of Gram and Ziehl Neelsen method are also used for the explanation of morphological structure of bacteria microorganism in conjunction with the following stain, malachite green, and methylene blue.
MALACHITE PREPARATION
Malachite green 1g
Distilled water 100ml
Dissolved the dye in a distilled water and filter record date and label.
PROCEDURE
Apply the stain for 20-30 second was with water blot carefully and dry by gentle heat.
apply oil immersion and view with light filed microscope.
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