POLYCHROME METHYLENE BLUE

The stain has a similarity with loeffers alkaline methylene blue. They are used for demonstration of Anthrax bacilli structure in the blood.

Preparation. the same with loeffer alkaline methylene blue. After that distribute in the battle, record date label.

Shake frequently before it can be ready for used, it takes several months, but it can be foster by treating with chemical.

Procedure: is the same of that of loefler alkaline methylene blue.

ALBERT STAIN

This stain is use for demonstration of corynebacterium diphtheriae, in microbiology laboratory. It has two solutions one and two.

 SOLUTION PREPARATION

Toluidine blue                   1.5g

Malachite green                2 .0g

Ethyl acetic acid              10ml

Ethyl alcohol -95%           20ml

Distilled water                  100ml

METHOD OF PREPARATION

First dissolved tuluidine blue, and malachite alcohol then add water and acetic acid, leave the solution at room temperature for 24h and filter, record date label and store.

2 SOLUTIONS

Iodine                           6g

Potassium iodide          9g

Distilled water             900ml

METHOD OF PREPARATION

Dissolved potassium iodide into 50ml of water and iodine dissolved and make up the total volume by adding water.

PROCEDURE

1 prepares a smear from loeffer serum culture between 18-24h of the test organism and allowed to dry and fix with gentle heat.

2 Apply solutions one for 3-5 minutes

3 Wash with water and blot carefully.

4 Apply solutions 2 for 1 minute.

5 Wash with water and blot carefully and dry by gentle heat.

6 Take to microscopy for examination.

Result: Granule stained bluish black the remaining organism stained green.

Pugh Stain: This stained is also used for the examination of the structure of Carynebacterium diptheriae.

SOLUTION

Taluidine blue                          1g

Ethyl alcohol absolute             20ml

Acetic acid glacial                   50ml

PREPARATION

Mix acetic acid with distilled water and add toliudine blue and dissolved in ethyl alcohol filter record date and label and store.

PROCEDURE

1 Prepare a smear from serum culture between 18-24hour of loeffer test organism allowed to dry and fix by gentile heat.

2 Apply solutions for 1-3 minute

3 Wash with water blot carefully and dry by gentle heating.

Examine with microscope.