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ISBN-13 978-0-521-67631-1
ISBN-13 978-0-511-34842-6
© Monica Cheesbrough 2000, 2006
Every effort has been made in preparing this book to provide accurate and up-to-date
information which is in accord with accepted standards and practice at the time of
publication. Nevertheless, the authors, editors and publisher can make no warranties that
the information contained herein is totally free from error, not least because clinical
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from the use of material contained in this book. Readers are strongly advised to pay careful
attention to information provided by the manufacturer of any drugs or equipment that
they plan to use.
2006
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Published in the United States of America by Cambridge University Press, New York
Click here to download Monica Cheessbrough Medical laboraotry part 2
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7.2 Features and classification of microorganisms of medical importance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9–35
7.3 Microscopical techniques used in microbiology . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35–45
7.4 Culturing bacterial pathogens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45–62
7.5 Biochemical tests to identify bacteria . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62–70
7.6 Examination of sputum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71–76
7.7 Examination of throat and mouth specimens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76–79
7.8 Examination of pus, ulcer material and skin specimens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80–85
7.9 Examination of effusions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85–90
7.10 Examination of urogenital specimens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90–97
7.11 Examination of faecal specimens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97–105
7.12 Examination of urine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105–115
7.13 Examination of cerebrospinal fluid (c.s.f.) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116–124
7.14 Culturing blood . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124–130
7.15 Examination of semen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130–132
7.16 Antimicrobial susceptibility testing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132–143
7.17 Water-related diseases and testing of water supplies. . . . . . .. . . . . . . 143–157
7.18 Summary of the clinical and laboratory features of microorganisms
Bacterial pathogens . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . 157–234
Fungal pathogens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 234–247
Viral pathogens. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 248–266
COLOUR SECTION. . . . . . . . . . . between p. 266 and p. 267
Chapter 8 Haematological tests
8.1 Haematology in district laboratories and quality assurance . . . . . . . 268–271
8.2 Functions of blood, haematopoiesis and blood disorders . . . . . . . .. . 271–295
8.3 Collection of blood . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295–299
8.4 Measurement of haemoglobin . . . . . . . . . . .. . . . . . . . . . .. . . . . . . . . . . . . 299–309
8.5 PCV and red cell indices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 309–313
8.6 Counting white cells and platelets . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 313–319
8.7 Blood films . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 319–329
8.8 Erythrocyte sedimentation rate. . . . . . . . . .. . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . 329–331
8.9 Reticulocyte count. Methaemoglobin reduction test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . 331–334
8.10 Investigation of sickle cell disease . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 334–340
8.11 Investigation of bleeding disorders . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . 340–347
Chapter 9 Blood transfusion tests
9.1 Blood transfusion services at district level and quality assurance . . .. . . 348–351
9.2 Blood donation and storage of blood . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 352–361
9.3 Blood grouping. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 362–369
9.4 Compatibility testing . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . .. . 369–378
Recommended Book. . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . . . . . 379
Details of Part 1 . . . . . . . . . . …… . . . . . . . . . . . . . ……… . . . . 380
Appendix I
Preparation of reagents and culture media . . . . . . . . . . . . . . . . ………………….. . . . . . . . . . . . . . 382–407
Appendix II
Useful addresses . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . 410–416
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ……. . . . . 417–434
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