Fluid Examination in the Laboratory
Macroscopy, such as the appearance of the fluid should be noted. Transfer the fluid to clean sterile centrifuge container or tube, and centrifuge the fluid for 5 minute at 3000rpm. Discard the supernatant, then the deposit into two prepared blood agar plate, one in an aerobic atmosphere and the other in an anaerobic atmosphere at 37ºC. stain the smear using gram staining, Ziehl-neelsen, Auramine-phenol Leishman staining method and view via microscope, and identify the organism seen. But in case of suspecting mycobacterium turberculosis, then the deposit should be inoculated directly in a two Lowestern-Jensen slopes. Don’t forget to treat sample suspected of mycobacterium terberculosis like sputum specimen.
Below are some pathogen that may be identify in the culture which are;
Mycobacterium turberculosis
Streptococcus pneumonia
Anaerobic streptococci
Staphylococcus aureus
Viridans streptococci
Haemolytic streptococci
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