LIQUID MEDIA SPECIMEN PREPARATION STEP
- Sterilized wire loop by passing through flame, allowed it to red hot bring it out and allowed it to cool before picking a colony
- Take a loop full of culture; transfer to a clean grease free glass slide spread the sample with the loop to form a thick film of a fluid.
- sterilized the wire loop
- Leave the film to air dry without heating and pass through the flame 3 time to kill the bacteria and fixed it to a slide.
- the slide should be allowed to cool
- stain with the right stain
- examine with microscopy, by microscopic procedure.
SOLID MEDIA SPECIMEN PREPARATION STEP
- sterilized the wire loop
- place a drop of distilled water on a grease free clean slide
- sterilized the loop
- with wire loop transfer a loopful colony to be examine on a slide
- mix it with the water to give you a uniformed films, sterilized the wire loop
- Allowed the film to air dry and fix with the appropriate stain and take it to microscope for examination via microscopic procedure.
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