Smear From cultural plate
- Use a clean grease free glass slide
- Mark the slide with a written material such as diamond grease or pencil, but pencil is easily rube away.
- Use only one smear per slide and keep the slides well transfer of acid fast organism onto another slide. Throw the slide away after used
- Make fairly heavy smear from liquid cultures and thin smear from solid media culture.
- Aseptic precaution must be observed during manipulation of the culture tube or plate.
- Do not use water taken from rubber attached to the taps for making smear as organism may be transfer from, the rubber.
- Disinfect spill immediately.
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