Chromatography is an analytical method widely employed in the field of chemistry and biology for separation.  The method is used mostly with a natural occurring substance, because most of this naturally occurring substance are mixture of substance that can only be separated or analyzed using any of the chromatographic method of analysis. On this post we only going to discuss theoretical aspect of chromatography.

History of Chromatography

Chromatography was introduce by a Russian Botanist named Micharl Iswett. Chromatography has two phase which are;

Stationary phase and mobile phase.

The separating mechanism work by the distribution of the separating substance between the stationary and moving phase. This separating method was used initially on plant colour separation, colour in a Greek word is known as chromos, which is where chromatography derived it name from. But now the separating method is employed in an extensive method for separation and purification of colour and colourless organic substance. Separation of a component of a substance in chromatography depend on their distribution difference between two non-miscible phases. One of the phase, either solid or liquid phase is fixed in the system. While other phase, mobile phase, fluid is streamed via the chromatographic system.

Point to note is that the mobile phase of liquid phase depend on the type chromatography used, on gas chromatography, mobile phase is a gas, while in liquid chromatography the mobile phase is liquid.

The substance subjected for analysis is distributed between mobile and the stationary phase, the substance in the stationary phase are retained there, while substance on the mobile phase migrate with the velocity of the phase. But each of the substance have different net migration, the different take place as a result of different distribution of particular analyzes mean residence time in the stationary phase.

We have different type of chromatography such as;

  1. Paper chromatography
  2. Thin-layer chromatography
  3. Column chromatography
  4. Gas chromatography
  5. High performance liquid chromatography

Each of this type of chromatography will be discus in a separate post. In other not to miss it, please subscribe to our newsletter so that we can keep you updated anytime we publish new post.

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