Since exposing many of these microorganism to free oxygen will kill them, a special precaution should be adhere to when handling the microorganism in other to prevent coursing dead to then, doe to exposure to the external environment. The major thing that course death to the microorganism is how they are handled in the external environment because of the external environment consist of a mixture of oxygen gas in the atmosphere, even thought the handling cannot be avoided, it should not be handle for long period of time in the external environment because the oxygen in the atmosphere will kill the micro organism. And it necessary to be regenerating atmospheric CO2 in an anaerobic jar containing culture, and specimen for culture should be place in the anaerobic jar immediately when collected from the patient.

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