Genitourinary Swab Sample

These swabs are mostly collected from genital organ, the sample is specifically collected incase of suspicion of genital infection, the suspected microorganism that cause genital infection;

Trichomoniasis

Puerperal sepsis

Gonorrhea

Escherichia coli

Staphylococcus  aureus e.t.c.

These swabs is usually incorporated in amies or Stuarts transport medium if their any possibility of delay in sending the sample to the laboratory. But it should be born in mind that for isolation of chlamydia, then special media is needed. Normal flora that may be seen I the sample are;

Feacal streptococci

Diphtheroids

Fusiform bacilli

Staphylococci

Coliform bacilli.

Method For Isolation

The sample should be inoculated in a specific prepared plate medium at 35ºC in an anaerobic environment with 10% CO₂ concentration. With the sample prepared a smear after the inoculation and stain with Gram staining techniques.

But for examination of vaginal species like Trichomonas Vaginalis, then prepared wet preparation of smear, follow the necessary step concerning wet preparation and examine with microscope. The next day examine and isolate the microorganism found on the culture plate, reincubate the plate again to next day. Below are some of the organism that may be likely seen in the culture plate;

Neisseria gonorrhoeae

Clostridium perfringens

Gardnerrela vaginalis

Candida albicans

Staphylococcus aureus

And some other anaerobic microorganism.

Eye Swabs : these sample is obtain from eyes for microbiological examination. The sample from are usually collected and, where the culturing media is already prepared waiting the sample to be inoculated, so at such the sample is often inoculated instantaneously, the instant inoculation is carry out in order to prevent any enzymatic action that may result of killing the present organism.

Method Of culturing

Inoculate one of the specific medium (blood agar, chocolate agar, MacConkey agar, and sabouraud agar) in a CO₂ at 35ºC anaerobically. Examine and identify any colonies of microorganism growth next of the inoculation. Some of the organism that may likely growth include;

Haemophilus influenza

Diptheroids

Haemolytic streptococcus

Staphylococcus aureus

Neisseria gonorrhea

Pneumococci