ESTIMATION OF PLASMA/SERUM BIOCARBONATE

REAGENT

COMPOSITION

Saline 1g

Distilled water 100ml

PREPARATION

Dissolved 1g of Sodium chloride in 100ml of 100ml water

COMPOSITION

Distilled water

Sodium hydroxide

PREPARATION

Dissolve 0.1NaOH in distilled water, make 1: 10 dilution.

COMPOSITION

Phenol red 0.1g

0.5NaOH (sodium hydroxide) 6.0ml

Distilled water 100ml

PREPARATION

Dissolved 0.1g of Phenol red in 6.0ml 0.5NaOH (sodium hydroxide) and dilute it with 100ml of distilled water

METHOD

Set up two tube as showing in the table below

REAGENT	CONTROL TUBE	TEST TUBE
Serum	0.1ml	0.1ml
Normal saline	5.0ml	4.0ml
Indicator solution	2 drop	2 drop
0.01NHCL	1.0ml	1.0ml
0.01NaOH		Drop by drop till the colour change from yellow to red

STEP 1

Preparation of a control tubes as follow

Add 5.0ml of normal saline in a control tube; add 0.1ml of serum to the tube, add 2 drop of phenol red indicator mix very well.

The colour should correspond to the end point of the titration

STEP 2

Add 4.0ml of normal saline in a test tube, add 1.0ml of 0.0NHCL follow with 0.1ml of serum, add 2 drop of phenol red indicator.

Titrate against 0.0N NaOH till the colour change from yellow to red.

Then compare the colour of the test tube with the control tube. See table above for practical example.

NB: the reading is in ml si unit

CALCULATION

(1-Xml of 0.01N NaOH requird) x 100

Naijablog